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anti glua2 rabbit antibody  (Proteintech)


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    Proteintech anti glua2 rabbit antibody
    Anti Glua2 Rabbit Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+glua2/Glutamate+receptor+2+Antibody/pm40857409-211-59-63
    Average 94 stars, based on 83 article reviews
    anti glua2 rabbit antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: Stress dynamically modulates neuronal autophagy to gate depression onset.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies:for western blot, rabbit anti-p62 (1:2000, #PM045, MBL), rabbit anti-Beclin-1(1:1000, #3495, CST), mouse antiAtg7 (1:1000, #67341-1-Ig, Proteintech), mouse anti-ubiquitin (1:1000, #3936, CST), rabbit anti-p-AMPKα (Thr172) (1:1000, #2535, CST), rabbit anti-p-mTOR (1:1000, #ab137133, Abcam), rabbit anti-p-p70 S6 Kinase 1-T421/S424 (1:1000, #AP0502, Abclonal), rabbit anti-p-Akt (1:1000, #4058, CST), rabbit anti-Cleaved Caspase-3 (1:1000, #9664, CST), rabbit anti-GluA1 (1:1000, #ab31232, Abcam), rabbit anti-GluA2 (1:1000, #11994-1-AP, Proteintech), rabbit anti-GluN1 (1:1000, #ab109182, Abcam), rabbit Anti-GABAA Receptor alpha 1 (1:1000, #ab252430, Abcam), mouse anti-β-actin (1:5000, #MA5-15739, Thermo Fisher Scientific), or rabbit anti-α-tubulin (1:2000, #11224-1-AP, Proteintech); for immunohistochemistry experiment: mouse anti-LC3, 1:200, #A17424, Abclonal; rabbit antiNeuN, 1:500, #A19086, Abclonal; rabbit anti-LC3, 1:1000, #ab192890, Abcam; mouse anti-PSD95, 1:500, #ab13552, Abcam; or guinea pig anti-NeuN, 1:500, #ABN90, Sigma-Aldrich; mouse anti-LC3, 1:100, #83506, CST. .. Secondary antibodies, for western blot, including anti-mouse IgG, HRP-linked antibody (1:10000, #31430, Thermo Fisher Scientific) and anti-rabbit IgG, HRP-linked antibody (1:5000, #31460, Thermo Fisher Scientific) ; goat anti-mouse, 1:100, #AS008, Abclonal or 1:1000, #A-21235, Thermo Fisher Scientific; goat anti-rabbit, 1:1000, #AS060, Abclonal, or 1:1000, #A-11008, Thermo Fisher Scientific; goat anti-guinea pig, 1:1000, #A-21435, Thermo Fisher Scientific; goat anti-mouse, 1:1,000, #A11001, Invitrogen.

    Flow Cytometry:

    Article Title: Stress dynamically modulates neuronal autophagy to gate depression onset.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies:for western blot, rabbit anti-p62 (1:2000, #PM045, MBL), rabbit anti-Beclin-1(1:1000, #3495, CST), mouse antiAtg7 (1:1000, #67341-1-Ig, Proteintech), mouse anti-ubiquitin (1:1000, #3936, CST), rabbit anti-p-AMPKα (Thr172) (1:1000, #2535, CST), rabbit anti-p-mTOR (1:1000, #ab137133, Abcam), rabbit anti-p-p70 S6 Kinase 1-T421/S424 (1:1000, #AP0502, Abclonal), rabbit anti-p-Akt (1:1000, #4058, CST), rabbit anti-Cleaved Caspase-3 (1:1000, #9664, CST), rabbit anti-GluA1 (1:1000, #ab31232, Abcam), rabbit anti-GluA2 (1:1000, #11994-1-AP, Proteintech), rabbit anti-GluN1 (1:1000, #ab109182, Abcam), rabbit Anti-GABAA Receptor alpha 1 (1:1000, #ab252430, Abcam), mouse anti-β-actin (1:5000, #MA5-15739, Thermo Fisher Scientific), or rabbit anti-α-tubulin (1:2000, #11224-1-AP, Proteintech); for immunohistochemistry experiment: mouse anti-LC3, 1:200, #A17424, Abclonal; rabbit antiNeuN, 1:500, #A19086, Abclonal; rabbit anti-LC3, 1:1000, #ab192890, Abcam; mouse anti-PSD95, 1:500, #ab13552, Abcam; or guinea pig anti-NeuN, 1:500, #ABN90, Sigma-Aldrich; mouse anti-LC3, 1:100, #83506, CST. .. Secondary antibodies, for western blot, including anti-mouse IgG, HRP-linked antibody (1:10000, #31430, Thermo Fisher Scientific) and anti-rabbit IgG, HRP-linked antibody (1:5000, #31460, Thermo Fisher Scientific) ; goat anti-mouse, 1:100, #AS008, Abclonal or 1:1000, #A-21235, Thermo Fisher Scientific; goat anti-rabbit, 1:1000, #AS060, Abclonal, or 1:1000, #A-11008, Thermo Fisher Scientific; goat anti-guinea pig, 1:1000, #A-21435, Thermo Fisher Scientific; goat anti-mouse, 1:1,000, #A11001, Invitrogen.

    Magnetic Resonance Imaging:

    Article Title: Stress dynamically modulates neuronal autophagy to gate depression onset.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies:for western blot, rabbit anti-p62 (1:2000, #PM045, MBL), rabbit anti-Beclin-1(1:1000, #3495, CST), mouse antiAtg7 (1:1000, #67341-1-Ig, Proteintech), mouse anti-ubiquitin (1:1000, #3936, CST), rabbit anti-p-AMPKα (Thr172) (1:1000, #2535, CST), rabbit anti-p-mTOR (1:1000, #ab137133, Abcam), rabbit anti-p-p70 S6 Kinase 1-T421/S424 (1:1000, #AP0502, Abclonal), rabbit anti-p-Akt (1:1000, #4058, CST), rabbit anti-Cleaved Caspase-3 (1:1000, #9664, CST), rabbit anti-GluA1 (1:1000, #ab31232, Abcam), rabbit anti-GluA2 (1:1000, #11994-1-AP, Proteintech), rabbit anti-GluN1 (1:1000, #ab109182, Abcam), rabbit Anti-GABAA Receptor alpha 1 (1:1000, #ab252430, Abcam), mouse anti-β-actin (1:5000, #MA5-15739, Thermo Fisher Scientific), or rabbit anti-α-tubulin (1:2000, #11224-1-AP, Proteintech); for immunohistochemistry experiment: mouse anti-LC3, 1:200, #A17424, Abclonal; rabbit antiNeuN, 1:500, #A19086, Abclonal; rabbit anti-LC3, 1:1000, #ab192890, Abcam; mouse anti-PSD95, 1:500, #ab13552, Abcam; or guinea pig anti-NeuN, 1:500, #ABN90, Sigma-Aldrich; mouse anti-LC3, 1:100, #83506, CST. .. Secondary antibodies, for western blot, including anti-mouse IgG, HRP-linked antibody (1:10000, #31430, Thermo Fisher Scientific) and anti-rabbit IgG, HRP-linked antibody (1:5000, #31460, Thermo Fisher Scientific) ; goat anti-mouse, 1:100, #AS008, Abclonal or 1:1000, #A-21235, Thermo Fisher Scientific; goat anti-rabbit, 1:1000, #AS060, Abclonal, or 1:1000, #A-11008, Thermo Fisher Scientific; goat anti-guinea pig, 1:1000, #A-21435, Thermo Fisher Scientific; goat anti-mouse, 1:1,000, #A11001, Invitrogen.

    Western Blot:

    Article Title: Stress dynamically modulates neuronal autophagy to gate depression onset.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies:for western blot, rabbit anti-p62 (1:2000, #PM045, MBL), rabbit anti-Beclin-1(1:1000, #3495, CST), mouse antiAtg7 (1:1000, #67341-1-Ig, Proteintech), mouse anti-ubiquitin (1:1000, #3936, CST), rabbit anti-p-AMPKα (Thr172) (1:1000, #2535, CST), rabbit anti-p-mTOR (1:1000, #ab137133, Abcam), rabbit anti-p-p70 S6 Kinase 1-T421/S424 (1:1000, #AP0502, Abclonal), rabbit anti-p-Akt (1:1000, #4058, CST), rabbit anti-Cleaved Caspase-3 (1:1000, #9664, CST), rabbit anti-GluA1 (1:1000, #ab31232, Abcam), rabbit anti-GluA2 (1:1000, #11994-1-AP, Proteintech), rabbit anti-GluN1 (1:1000, #ab109182, Abcam), rabbit Anti-GABAA Receptor alpha 1 (1:1000, #ab252430, Abcam), mouse anti-β-actin (1:5000, #MA5-15739, Thermo Fisher Scientific), or rabbit anti-α-tubulin (1:2000, #11224-1-AP, Proteintech); for immunohistochemistry experiment: mouse anti-LC3, 1:200, #A17424, Abclonal; rabbit antiNeuN, 1:500, #A19086, Abclonal; rabbit anti-LC3, 1:1000, #ab192890, Abcam; mouse anti-PSD95, 1:500, #ab13552, Abcam; or guinea pig anti-NeuN, 1:500, #ABN90, Sigma-Aldrich; mouse anti-LC3, 1:100, #83506, CST. .. Secondary antibodies, for western blot, including anti-mouse IgG, HRP-linked antibody (1:10000, #31430, Thermo Fisher Scientific) and anti-rabbit IgG, HRP-linked antibody (1:5000, #31460, Thermo Fisher Scientific) ; goat anti-mouse, 1:100, #AS008, Abclonal or 1:1000, #A-21235, Thermo Fisher Scientific; goat anti-rabbit, 1:1000, #AS060, Abclonal, or 1:1000, #A-11008, Thermo Fisher Scientific; goat anti-guinea pig, 1:1000, #A-21435, Thermo Fisher Scientific; goat anti-mouse, 1:1,000, #A11001, Invitrogen.

    Immunohistochemistry:

    Article Title: Stress dynamically modulates neuronal autophagy to gate depression onset.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies:for western blot, rabbit anti-p62 (1:2000, #PM045, MBL), rabbit anti-Beclin-1(1:1000, #3495, CST), mouse antiAtg7 (1:1000, #67341-1-Ig, Proteintech), mouse anti-ubiquitin (1:1000, #3936, CST), rabbit anti-p-AMPKα (Thr172) (1:1000, #2535, CST), rabbit anti-p-mTOR (1:1000, #ab137133, Abcam), rabbit anti-p-p70 S6 Kinase 1-T421/S424 (1:1000, #AP0502, Abclonal), rabbit anti-p-Akt (1:1000, #4058, CST), rabbit anti-Cleaved Caspase-3 (1:1000, #9664, CST), rabbit anti-GluA1 (1:1000, #ab31232, Abcam), rabbit anti-GluA2 (1:1000, #11994-1-AP, Proteintech), rabbit anti-GluN1 (1:1000, #ab109182, Abcam), rabbit Anti-GABAA Receptor alpha 1 (1:1000, #ab252430, Abcam), mouse anti-β-actin (1:5000, #MA5-15739, Thermo Fisher Scientific), or rabbit anti-α-tubulin (1:2000, #11224-1-AP, Proteintech); for immunohistochemistry experiment: mouse anti-LC3, 1:200, #A17424, Abclonal; rabbit antiNeuN, 1:500, #A19086, Abclonal; rabbit anti-LC3, 1:1000, #ab192890, Abcam; mouse anti-PSD95, 1:500, #ab13552, Abcam; or guinea pig anti-NeuN, 1:500, #ABN90, Sigma-Aldrich; mouse anti-LC3, 1:100, #83506, CST. .. Secondary antibodies, for western blot, including anti-mouse IgG, HRP-linked antibody (1:10000, #31430, Thermo Fisher Scientific) and anti-rabbit IgG, HRP-linked antibody (1:5000, #31460, Thermo Fisher Scientific) ; goat anti-mouse, 1:100, #AS008, Abclonal or 1:1000, #A-21235, Thermo Fisher Scientific; goat anti-rabbit, 1:1000, #AS060, Abclonal, or 1:1000, #A-11008, Thermo Fisher Scientific; goat anti-guinea pig, 1:1000, #A-21435, Thermo Fisher Scientific; goat anti-mouse, 1:1,000, #A11001, Invitrogen.

    Saline:

    Article Title: Impaired Response to Mismatch Novelty in the Li 2+ -Pilocarpine Rat Model of TLE: Correlation with Hippocampal Monoaminergic Inputs.
    Article Snippet: .. These were then blocked for 1 h with either 3% BSA or 5% milk solution in Tris-buffered saline containing 1% Tween (TBST) and incubated overnight at 4 ◦C with rabbit anti-dopamine transporter (DAT, 1:2000, Proteintech Cat# 22524-1-AP, RRID:AB_2879116), mouse monoclonal anti-norepinephrine transporter (NET, 1:500, Atlas Antibodies Cat# AMAb91116, RRID:AB_2665806), rabbit anti-serotonin transporter (SERT, 1:1000, Proteintech Cat# 19559-1-AP; AB_2878590), rabbit anti-tyrosine hydroxylase (TH, 1:1000, Abcam Cat# Ab112; RRID:AB_297840), mouse monoclonal anti-gephyrin (1:3000, Synaptic Systems #147011, RRID:AB_2810215), rabbit anti-PSD95 (1:750, Cell Signalling Technology #2507, RRID:AB_561221), rabbit anti-GluA1 (1:4000, Millipore Cat# AB1504; RRID:AB_2113602), rabbit anti-GluA2 (1:1000, Proteintech Cat# 11994-1-AP; RRID: AB_2113725), mouse monoclonal anti-GluN1 (1:1000, Proteintech Cat# 67717-1-Ig; RRID: AB_2882906), rabbit anti-GluN2B (1:1000, Cell Signalling Technology Cat#4207; RRID: AB_1264223), rabbit anti-synaptophysin (1:7500, Synaptic Systems #101002, RRID:AB_887905), and either mouse monoclonal anti-β-actin (1:5000, Proteintech #60008-1- Ig, RRID: AB_2289225) or rabbit polyclonal anti-α-tubulin (1:4000, Proteintech #PT112241-AP, RRID: AB_ 2210206) primary antibodies. ..

    Article Title: Impaired Response to Mismatch Novelty in the Li 2+ -Pilocarpine Rat Model of TLE: Correlation with Hippocampal Monoaminergic Inputs
    Article Snippet: .. These were then blocked for 1 h with either 3% BSA or 5% milk solution in Tris-buffered saline containing 1% Tween (TBST) and incubated overnight at 4 °C with rabbit anti-dopamine transporter (DAT, 1:2000, Proteintech Cat# 22524-1-AP, RRID:AB_2879116), mouse monoclonal anti-norepinephrine transporter (NET, 1:500, Atlas Antibodies Cat# AMAb91116, RRID:AB_2665806), rabbit anti-serotonin transporter (SERT, 1:1000, Proteintech Cat# 19559-1-AP; AB_2878590), rabbit anti-tyrosine hydroxylase (TH, 1:1000, Abcam Cat# Ab112; RRID:AB_297840), mouse monoclonal anti-gephyrin (1:3000, Synaptic Systems #147011, RRID:AB_2810215), rabbit anti-PSD-95 (1:750, Cell Signalling Technology #2507, RRID:AB_561221), rabbit anti-GluA1 (1:4000, Millipore Cat# AB1504; RRID:AB_2113602), rabbit anti-GluA2 (1:1000, Proteintech Cat# 11994-1-AP; RRID: AB_2113725), mouse monoclonal anti-GluN1 (1:1000, Proteintech Cat# 67717-1-Ig; RRID: AB_2882906), rabbit anti-GluN2B (1:1000, Cell Signalling Technology Cat#4207; RRID: AB_1264223), rabbit anti-synaptophysin (1:7500, Synaptic Systems #101002, RRID:AB_887905), and either mouse monoclonal anti-β-actin (1:5000, Proteintech #60008-1-Ig, RRID: AB_2289225) or rabbit polyclonal anti-α-tubulin (1:4000, Proteintech #PT11224-1-AP, RRID: AB_ 2210206) primary antibodies. ..

    Article Title: Impaired response to mismatch novelty in the Li 2+ -pilocarpine rat model of TLE: correlation with hippocampal monoaminergic inputs
    Article Snippet: .. These were then blocked for 1 h with either 3% BSA or 5% milk solution in Tris-buffered saline containing 1% Tween (TBST) and incubated overnight at 4°C with rabbit anti dopamine transporter (DAT, 1:2000, Proteintech Cat# 22524-1-AP, RRID:AB_2879116), mouse monoclonal anti norepinephrine transporter (NET, 1:500, Atlas Antibodies Cat# AMAb91116, RRID:AB_2665806), rabbit anti serotonin transporter (SERT, 1:1000, Proteintech Cat# 19559-1-AP; AB_2878590), rabbit anti-tyrosine hydroxylase (TH, 1:1000, Abcam Cat# Ab112; RRID:AB_297840), mouse monoclonal anti-gephyrin (1:3000, Synaptic Systems #147011, RRID:AB_2810215), rabbit anti-PSD-95 (1:750, Cell Signalling Technology #2507, RRID:AB_561221), rabbit anti-GluA1 (1:4000, Millipore Cat# AB1504; RRID:AB_2113602), rabbit anti-GluA2 (1:1000, Proteintech Cat# 11994-1-AP; RRID: AB_2113725), mouse monoclonal anti-GluN1 (1:1000, Proteintech Cat# 67717-1-Ig; RRID: AB_2882906), rabbit anti GluN2B (1:1000, Cell Signalling Technology Cat#4207; RRID: AB_1264223), rabbit anti-synaptophysin (1:7500, Synaptic Systems #101002, RRID:AB_887905), and either mouse monoclonal anti-β-actin (1:5000, Proteintech #60008-1-Ig, RRID: AB_2289225) or rabbit polyclonal anti-α-tubulin (1:4000, Proteintech #PT11224-1-AP, RRID: AB_ 2210206) primary antibodies. .. After washing 3x for 10 min with TBST, the membranes were incubated for 1h with anti-rabbit IgG or anti-mouse IgG secondary antibody both conjugated with horseradish peroxidase (HRP) (Proteintech) at room temperature.

    Incubation:

    Article Title: Impaired Response to Mismatch Novelty in the Li 2+ -Pilocarpine Rat Model of TLE: Correlation with Hippocampal Monoaminergic Inputs.
    Article Snippet: .. These were then blocked for 1 h with either 3% BSA or 5% milk solution in Tris-buffered saline containing 1% Tween (TBST) and incubated overnight at 4 ◦C with rabbit anti-dopamine transporter (DAT, 1:2000, Proteintech Cat# 22524-1-AP, RRID:AB_2879116), mouse monoclonal anti-norepinephrine transporter (NET, 1:500, Atlas Antibodies Cat# AMAb91116, RRID:AB_2665806), rabbit anti-serotonin transporter (SERT, 1:1000, Proteintech Cat# 19559-1-AP; AB_2878590), rabbit anti-tyrosine hydroxylase (TH, 1:1000, Abcam Cat# Ab112; RRID:AB_297840), mouse monoclonal anti-gephyrin (1:3000, Synaptic Systems #147011, RRID:AB_2810215), rabbit anti-PSD95 (1:750, Cell Signalling Technology #2507, RRID:AB_561221), rabbit anti-GluA1 (1:4000, Millipore Cat# AB1504; RRID:AB_2113602), rabbit anti-GluA2 (1:1000, Proteintech Cat# 11994-1-AP; RRID: AB_2113725), mouse monoclonal anti-GluN1 (1:1000, Proteintech Cat# 67717-1-Ig; RRID: AB_2882906), rabbit anti-GluN2B (1:1000, Cell Signalling Technology Cat#4207; RRID: AB_1264223), rabbit anti-synaptophysin (1:7500, Synaptic Systems #101002, RRID:AB_887905), and either mouse monoclonal anti-β-actin (1:5000, Proteintech #60008-1- Ig, RRID: AB_2289225) or rabbit polyclonal anti-α-tubulin (1:4000, Proteintech #PT112241-AP, RRID: AB_ 2210206) primary antibodies. ..

    Article Title: Stress dynamically modulates neuronal autophagy to gate depression onset
    Article Snippet: .. The blots were incubated at 4 °C overnight with one of the following primary antibodies: rabbit anti-p62 (1:2,000, PM045, MBL), rabbit anti-Beclin-1 (1:1,000, 3495, CST), mouse anti-Atg7 (1:1,000, 67341-1-Ig, Proteintech), mouse anti-ubiquitin (1:1,000, 3936, CST), rabbit anti-p-AMPKα (Thr172) (1:1,000, 2535, CST), rabbit anti-p-mTOR (1:1,000, ab137133, Abcam), rabbit anti-p-p70S6 Kinase 1-T421/S424 (1:1,000, AP0502, ABclonal), rabbit anti-p-Akt (1:1,000, 4058, CST), rabbit anti-Cleaved Caspase-3 (1:1,000, 9664, CST), rabbit anti-GluA1 (1:1,000, ab31232, Abcam), rabbit anti-GluA2 (1:1,000, 11994-1-AP, Proteintech), rabbit anti-GluN1 (1:1,000, ab109182, Abcam), rabbit Anti-GABA A Receptor alpha 1 (1:1,000, ab252430, Abcam), mouse anti-β-actin (1:5,000, MA5-15739, Thermo Fisher Scientific) or rabbit anti-α-tubulin (1:2,000, 11224-1-AP, Proteintech). .. The next day, horseradish peroxidase (HRP)-conjugated secondary antibodies, including anti-mouse IgG, HRP-linked antibody (1:10,000, 31430, Thermo Fisher Scientific) and anti-rabbit IgG, HRP-linked antibody (1:5,000, 31460, Thermo Fisher Scientific) were revealed using an ECL kit (Immobilon Ultra Western HRP Substrate, MILLIPORE) for protein detection.

    Article Title: Impaired Response to Mismatch Novelty in the Li 2+ -Pilocarpine Rat Model of TLE: Correlation with Hippocampal Monoaminergic Inputs
    Article Snippet: .. These were then blocked for 1 h with either 3% BSA or 5% milk solution in Tris-buffered saline containing 1% Tween (TBST) and incubated overnight at 4 °C with rabbit anti-dopamine transporter (DAT, 1:2000, Proteintech Cat# 22524-1-AP, RRID:AB_2879116), mouse monoclonal anti-norepinephrine transporter (NET, 1:500, Atlas Antibodies Cat# AMAb91116, RRID:AB_2665806), rabbit anti-serotonin transporter (SERT, 1:1000, Proteintech Cat# 19559-1-AP; AB_2878590), rabbit anti-tyrosine hydroxylase (TH, 1:1000, Abcam Cat# Ab112; RRID:AB_297840), mouse monoclonal anti-gephyrin (1:3000, Synaptic Systems #147011, RRID:AB_2810215), rabbit anti-PSD-95 (1:750, Cell Signalling Technology #2507, RRID:AB_561221), rabbit anti-GluA1 (1:4000, Millipore Cat# AB1504; RRID:AB_2113602), rabbit anti-GluA2 (1:1000, Proteintech Cat# 11994-1-AP; RRID: AB_2113725), mouse monoclonal anti-GluN1 (1:1000, Proteintech Cat# 67717-1-Ig; RRID: AB_2882906), rabbit anti-GluN2B (1:1000, Cell Signalling Technology Cat#4207; RRID: AB_1264223), rabbit anti-synaptophysin (1:7500, Synaptic Systems #101002, RRID:AB_887905), and either mouse monoclonal anti-β-actin (1:5000, Proteintech #60008-1-Ig, RRID: AB_2289225) or rabbit polyclonal anti-α-tubulin (1:4000, Proteintech #PT11224-1-AP, RRID: AB_ 2210206) primary antibodies. ..

    Article Title: Impaired response to mismatch novelty in the Li 2+ -pilocarpine rat model of TLE: correlation with hippocampal monoaminergic inputs
    Article Snippet: .. These were then blocked for 1 h with either 3% BSA or 5% milk solution in Tris-buffered saline containing 1% Tween (TBST) and incubated overnight at 4°C with rabbit anti dopamine transporter (DAT, 1:2000, Proteintech Cat# 22524-1-AP, RRID:AB_2879116), mouse monoclonal anti norepinephrine transporter (NET, 1:500, Atlas Antibodies Cat# AMAb91116, RRID:AB_2665806), rabbit anti serotonin transporter (SERT, 1:1000, Proteintech Cat# 19559-1-AP; AB_2878590), rabbit anti-tyrosine hydroxylase (TH, 1:1000, Abcam Cat# Ab112; RRID:AB_297840), mouse monoclonal anti-gephyrin (1:3000, Synaptic Systems #147011, RRID:AB_2810215), rabbit anti-PSD-95 (1:750, Cell Signalling Technology #2507, RRID:AB_561221), rabbit anti-GluA1 (1:4000, Millipore Cat# AB1504; RRID:AB_2113602), rabbit anti-GluA2 (1:1000, Proteintech Cat# 11994-1-AP; RRID: AB_2113725), mouse monoclonal anti-GluN1 (1:1000, Proteintech Cat# 67717-1-Ig; RRID: AB_2882906), rabbit anti GluN2B (1:1000, Cell Signalling Technology Cat#4207; RRID: AB_1264223), rabbit anti-synaptophysin (1:7500, Synaptic Systems #101002, RRID:AB_887905), and either mouse monoclonal anti-β-actin (1:5000, Proteintech #60008-1-Ig, RRID: AB_2289225) or rabbit polyclonal anti-α-tubulin (1:4000, Proteintech #PT11224-1-AP, RRID: AB_ 2210206) primary antibodies. .. After washing 3x for 10 min with TBST, the membranes were incubated for 1h with anti-rabbit IgG or anti-mouse IgG secondary antibody both conjugated with horseradish peroxidase (HRP) (Proteintech) at room temperature.

    Article Title: Stress dynamically modulates neuronal autophagy to gate depression onset.
    Article Snippet: .. The blots were incubated at 4 °C overnight with one of the following primary antibodies: rabbit anti-p62 (1:2,000, PM045, MBL), rabbit anti-Beclin-1 (1:1,000, 3495, CST), mouse anti-Atg7 (1:1,000, 67341-1-Ig, Proteintech), mouse anti-ubiquitin (1:1,000, 3936, CST), rabbit anti-p-AMPKα (Thr172) (1:1,000, 2535, CST), rabbit anti-p-mTOR (1:1,000, ab137133, Abcam), rabbit anti-p-p70S6 Kinase 1-T421/S424 (1:1,000, AP0502, ABclonal), rabbit anti-p-Akt (1:1,000, 4058, CST), rabbit anti-Cleaved Caspase-3 (1:1,000, 9664, CST), rabbit anti-GluA1 (1:1,000, ab31232, Abcam), rabbit anti-GluA2 (1:1,000, 11994-1-AP, Proteintech), rabbit anti-GluN1 (1:1,000, ab109182, Abcam), rabbit Anti-GABAA Receptor alpha 1 (1:1,000, ab252430, Abcam), mouse anti-β-actin (1:5,000, MA5-15739, Thermo Fisher Scientific) or rabbit anti-α-tubulin (1:2,000, 11224-1-AP, Proteintech). .. The next day, horseradish peroxidase (HRP)-conjugated secondary antibodies, including anti-mouse IgG, HRP-linked antibody (1:10,000, 31430, Thermo Fisher Scientific) and anti-rabbit IgG, HRP-linked antibody (1:5,000, 31460, Thermo Fisher Scientific) were revealed using an ECL kit (Immobilon Ultra Western HRP Substrate, MILLIPORE) for protein detection.

    other:

    Article Title: Stress dynamically modulates neuronal autophagy to gate depression onset.
    Article Snippet: Subsequently, primary antibody was then added with incubation buffer and slides were left for 1 h at room temperature, followed by an overnight incubation with primary antibody (rabbit anti-GluA2 1:100, 11994-1-AP, Proteintech) at 4 °C.

    Article Title: Stress dynamically modulates neuronal autophagy to gate depression onset
    Article Snippet: Subsequently, primary antibody was then added with incubation buffer and slides were left for 1 h at room temperature, followed by an overnight incubation with primary antibody (rabbit anti-GluA2 1:100, 11994-1-AP, Proteintech) at 4 °C.



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    Alomone Labs rabbit anti glua2 gria2 alomone agc
    Rabbit Anti Glua2 Gria2 Alomone Agc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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